Composite

Part:BBa_K1891014:Design

Designed by: Xinyu Liu   Group: iGEM16_BNU-China   (2016-10-13)


P(3HB)(BBa_K934001) + HSP promotor (BBa_K873002)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2134
    Illegal BglII site found at 2959
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1440
    Illegal NgoMIV site found at 1511
    Illegal NgoMIV site found at 2111
    Illegal NgoMIV site found at 2423
    Illegal NgoMIV site found at 2702
    Illegal NgoMIV site found at 3354
    Illegal NgoMIV site found at 3376
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5220
    Illegal SapI site found at 961


Design Notes

We used Spe I and Pst I to do dual digestion in PSB1C3 which contained pBAD gene and recycled gene fragments in 3280bp, used Xbal I and Pst I to do dual digestion in PSB1C3 and recycled gene fragments in 4208bp.We use T4 ligase to link phaC1-A-B1 to the downstream of pBAD promoter, then transform the vectors to Trans 5α for plasmid extraction.


Source

We get parts BBa_K934001 and BBa_I0500 from the kits sent by iGEM HQ.

References